Methods for Kinetic Modeling of Temporally Resolved Hyperspectral Confocal Fluorescence Images
نویسندگان
چکیده
منابع مشابه
Systematic method for the kinetic modeling of temporally resolved hyperspectral microscope images of fluorescently labeled cells.
In this paper we report the application of a novel method for fitting kinetic models to temporally resolved hyperspectral images of fluorescently labeled cells to mathematically resolve pure-component spatial images, pure-component spectra, and pure-component reaction profiles. The method is demonstrated on one simulated image and two experimental cell images, including human embryonic kidney c...
متن کاملReconstruction from Fluorescence Confocal Microscopy Images
The fluorescence confocal microscopy is one of the most important tools in biomedical and pharmaceutic research. The main advantage of this technique over the traditional optical microscopy is the fact that it allows the selection of a thin cross-section of the sample by rejecting the visual information coming from the out of focus planes. Additionally, it uses fluorescence synthetic molecules ...
متن کاملThermally activated delayed fluorescence of fluorescein derivative for time-resolved and confocal fluorescence imaging.
Compared with fluorescence imaging utilizing fluorophores whose lifetimes are in the order of nanoseconds, time-resolved fluorescence microscopy has more advantages in monitoring target fluorescence. In this work, compound DCF-MPYM, which is based on a fluorescein derivative, showed long-lived luminescence (22.11 μs in deaerated ethanol) and was used in time-resolved fluorescence imaging in liv...
متن کاملHigh frame-rate fluorescence confocal angle-resolved linear dichroism microscopy.
Angle-resolved linear dichroism is a recent technique that exploits images recorded using an illumination field whose polarization angle is sequentially rotated during acquisition. It allows to retrieve orientation information of the fluorescent molecules, namely the average orientation angle and the amplitude of the fluctuations around this average. In order to boost up the acquisition speed w...
متن کاملApplication of image restoration methods for confocal fluorescence microscopy
The analysis of the three-dimensional structure of tissue, cells and cellular constituents play a major role in biomedical research. Three-dimensional images, acquired by confocal fluorescence microscopes play a key role in this analysis. However, the imaging properties of these microscopes give rise to diffraction-induced blurring phenomena. These distortions hamper subsequent quantitative ana...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Spectroscopy
سال: 2009
ISSN: 0003-7028,1943-3530
DOI: 10.1366/000370209787391950